xcell atf device  (Repligen Corp)

Journal: ACS Biomaterials Science & Engineering

Article Title: Self-Assembled Quercetin–Mecobalamin Nanoparticles Mitigate Ischemic Stroke Injury via Enhanced Neuroprotection and Microglial Modulation

doi: 10.1021/acsbiomaterials.5c01855

Figure Lengend Snippet: Qu-Me NPs attenuates MCAO-induced injury. (A,B) Open-field test performed 24 h post-MCAO shows improved locomotor activity in Qu- and Qu-Me NPs-treated mice compared with the MCAO and Me groups ( n = 6). (C,D) Y-maze test demonstrates ameliorated cognitive function in the Qu- and Qu-Me NPs-treated mice compared with the MCAO and Me groups ( n = 6). (E) Modified neurological severity scores (mNSS) for each group (Sham, MCAO, Me, Qu, and Qu-Me NPs) evaluated at 24 h post-MCAO ( n = 6). (F–J) Western blot analysis illustrates reduced expression of ER stress markers (ATF-6, CHOP) and pro-inflammatory cytokines (IL-1β, IL-6) in the Qu and Qu-Me NP groups compared with MCAO ( n = 3). Results are presented as averages ±SD * p < 0.05; ** p < 0.01; *** p < 0.001; ns, not significant. Analysis was performed using one-way ANOVA followed by Dunnett multiple-comparisons test for mean differences.

Article Snippet: Proteins were separated by SDS-PAGE, transferred to PVDF membranes, and incubated overnight with specific primary antibodies against β-actin (1/5000, ab170325, Abcam), ATF-6 (1:1000, A0202, ABclonal), CHOP (1:1000, A21902 , ABclonal), IL-1β (1/1000, 16806-1-AP, Proteintech), IL-6, pannexin1 (1/1000, ab124969, Abcam), and DDIT4 (1:1000, ab191871, Abcam).

Techniques: Activity Assay, Modification, Western Blot, Expressing